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If you only want to transcribe a portion of your file, you can set the audio_start_from and the audio_end_at parameters in your transcription config. 
import assemblyai as aai

aai.settings.api_key = "<YOUR_API_KEY>"

# audio_file = "./local_file.mp3"
audio_file = "https://assembly.ai/wildfires.mp3"

config = aai.TranscriptionConfig(
  audio_start_from=5000,  # The start time of the transcription in milliseconds
  audio_end_at=15000  # The end time of the transcription in milliseconds
)

transcript = aai.Transcriber(config=config).transcribe(audio_file)

if transcript.status == "error":
  raise RuntimeError(f"Transcription failed: {transcript.error}")

print(transcript.text)